Hotspot

trnS-GCU-trnG-CGA

LSC · 8784-9571 · 788 bp

Back to Torenia

Pi 0.017
MarkerSeek score 62.20
Primer available yes
Label trnS-GCU-trnG-CGA

Feature Metadata

label_name
trnS-GCU-trnG-CGA
feature_type
igs
region
LSC
length_bp
788
variable_sites
49
pi
0.017
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.89
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-CGA_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 770-857 bp 1.0 60.6
trnS-GCU-trnG-CGA_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 769-856 bp 1.0 60.6
trnS-GCU-trnG-CGA_p3 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 808-895 bp 1.0 60.5
trnS-GCU-trnG-CGA_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 807-894 bp 1.0 60.5
trnS-GCU-trnG-CGA_p5 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCTTTAACT 50.0 40.0 58.2 54.5 862-949 bp 1.0 59.1