Hotspot

trnK-UUU-rps16

LSC · 4466-5321 · 856 bp

Back to Torenia

Pi 0.0147
MarkerSeek score 65.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
856
variable_sites
55
pi
0.0147
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCCTTGAAAAAGGGGCTCAA 50.0 45.0 58.2 57.2 978-999 bp 1.0 77.8
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGGGCTCA 50.0 45.0 58.2 57.2 979-1000 bp 1.0 77.8
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCCTTGAAAAAGGGGCTCAA 50.0 45.0 58.2 57.2 977-998 bp 1.0 77.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGGGCTCA 50.0 45.0 58.2 57.2 978-999 bp 1.0 77.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGGGCTCAACC 50.0 50.0 58.2 57.2 975-996 bp 1.0 77.4