Hotspot

rrn16-trnV-GAC

Genome · 138049-139220 · 1172 bp

Back to Tordylium

Pi 0.163
MarkerSeek score 79.30
Primer available yes
Label rrn16-trnV-GAC

Feature Metadata

label_name
rrn16-trnV-GAC
feature_type
igs
region
Genome
length_bp
1172
variable_sites
186
pi
0.163
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnV-GAC_p1 CCTCAGCCTACGGGGTATTA GTACCGTGAGAGAAGCAAGG 55.0 55.0 58.0 58.0 1244-1911 bp 0.666667 71.5
rrn16-trnV-GAC_p2 CATTGCGGAAAATTCCCCAC GTACCGTGAGAGAAGCAAGG 50.0 55.0 58.0 58.0 1418-2085 bp 0.666667 71.4
rrn16-trnV-GAC_p3 CCTCAGCCTACGGGGTATTA TGAGAGAAGCAAGGAGGTCA 55.0 50.0 58.0 58.0 1238-1905 bp 0.666667 71.4
rrn16-trnV-GAC_p4 CCTCAGCCTACGGGGTATTA CCGGCCTAGTGAGTCTTTTC 55.0 55.0 58.0 58.0 2012 bp 0.333333 53.2
rrn16-trnV-GAC_p5 CATTGCGGAAAATTCCCCAC CCGGCCTAGTGAGTCTTTTC 50.0 55.0 58.0 58.0 2186 bp 0.333333 53.2