Hotspot

ndhF-rpl32

Genome · 112303-113246 · 944 bp

Back to Tordylium

Pi 0.0524

MarkerSeek score 82.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 944
variable_sites: 69
pi: 0.0524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCGGGTCGATCAAGTAAC	AGACTCCCCCATCTCCAAAA	50.0	50.0	58.0	57.9	1655-1741 bp	1.0	87.5
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	AGACTCCCCCATCTCCAAAA	50.0	50.0	58.0	57.9	1883-1969 bp	1.0	86.7
ndhF-rpl32_p3	AAGCGGGTCGATCAAGTAAC	AAAAGACTCCCCCATCTCCA	50.0	50.0	58.0	57.9	1658-1676 bp	0.666667	71.9
ndhF-rpl32_p4	AAGCGGGTCGATCAAGTAAC	AAAGACTCCCCCATCTCCAA	50.0	50.0	58.0	57.9	1657-1675 bp	0.666667	71.9
ndhF-rpl32_p5	AAGCGGGTCGATCAAGTAAC	AAGACTCCCCCATCTCCAAA	50.0	50.0	58.0	57.9	1656-1674 bp	0.666667	71.9