Hotspot

trnV-GAC-rrn16

Genome · 98340-99511 · 1172 bp

Back to Tordylium

Pi 0.163

MarkerSeek score 79.30

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 1172
variable_sites: 186
pi: 0.163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	GTACCGTGAGAGAAGCAAGG	CCTCAGCCTACGGGGTATTA	55.0	55.0	58.0	58.0	1244-2380 bp	1.0	91.9
trnV-GAC-rrn16_p2	GTACCGTGAGAGAAGCAAGG	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	1418-2554 bp	1.0	91.9
trnV-GAC-rrn16_p3	TGAGAGAAGCAAGGAGGTCA	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	1238-2374 bp	1.0	91.8
trnV-GAC-rrn16_p4	CCGGCCTAGTGAGTCTTTTC	CCTCAGCCTACGGGGTATTA	55.0	55.0	58.0	58.0	2012-2470 bp	0.666667	75.6
trnV-GAC-rrn16_p5	CCGGCCTAGTGAGTCTTTTC	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	2186-2644 bp	0.666667	74.9