Hotspot

trnM-CAU-atpE

Genome · 51976-52162 · 187 bp

Back to Tordylium

Pi 0.0588

MarkerSeek score 76.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: Genome
length_bp: 187
variable_sites: 16
pi: 0.0588
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CGGAACTAGTTGGTGTGTCC	50.0	55.0	58.4	58.2	262-269 bp	1.0	79.9
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	GGAACTAGTTGGTGTGTCCG	50.0	55.0	58.4	58.2	261-268 bp	1.0	79.9
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.4	58.1	318-325 bp	1.0	79.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	320-327 bp	1.0	79.5
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.4	58.2	319-326 bp	1.0	78.9