Hotspot

trnE-UUC-trnT-GGU

Genome · 29994-30775 · 782 bp

Back to Tordylium

Pi 0.0596

MarkerSeek score 82.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 782
variable_sites: 64
pi: 0.0596
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GAATGGCGAAACGAAAAGGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1319-1377 bp	1.0	88.7
trnE-UUC-trnT-GGU_p2	GGCGAAACGAAAAGGGAAAG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1315-1373 bp	1.0	88.5
trnE-UUC-trnT-GGU_p3	GAATGGCGAAACGAAAAGGG	TACCGCTGAGTTAAAAGGGC	50.0	50.0	58.0	57.9	1284-1342 bp	1.0	88.0
trnE-UUC-trnT-GGU_p4	GAATGGCGAAACGAAAAGGG	TGGTGATTCCAGAGACGACT	50.0	50.0	58.0	58.1	1582-1640 bp	1.0	87.7
trnE-UUC-trnT-GGU_p5	GGCGAAACGAAAAGGGAAAG	TGGTGATTCCAGAGACGACT	50.0	50.0	58.0	58.1	1578-1636 bp	1.0	87.5