Hotspot

trnS-GCU-trnG-UCC

Genome · 7536-8105 · 570 bp

Back to Tordylium

Pi 0.0444
MarkerSeek score 79.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
Genome
length_bp
570
variable_sites
36
pi
0.0444
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA GGGGGTTACTCGAACGAATC 45.0 55.0 58.0 58.1 598-657 bp 1.0 87.7
trnS-GCU-trnG-UCC_p2 GCTTTAGTCCACTCAGCCAT GGGGGTTACTCGAACGAATC 50.0 55.0 57.6 58.1 584-643 bp 1.0 84.8
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA AGGGGGTTACTCGAACGAAT 45.0 50.0 58.0 58.4 599-658 bp 1.0 84.7
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC GGGGGTTACTCGAACGAATC 55.0 55.0 58.5 58.1 579-638 bp 1.0 84.0
trnS-GCU-trnG-UCC_p5 ATTAGCAATCCGCCGCTTTA GGGGTTACTCGAACGAATCA 45.0 50.0 58.0 57.3 597-656 bp 1.0 82.9