Hotspot

trnK-UUU-rps16

Genome · 4112-4828 · 717 bp

Back to Tordylium

Pi 0.04
MarkerSeek score 79.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
Genome
length_bp
717
variable_sites
42
pi
0.04
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AGGAACTGTTCGGGATCTTT 50.0 45.0 58.0 56.5 841-859 bp 1.0 72.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 858-876 bp 1.0 72.3
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 857-875 bp 1.0 72.3
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 797-815 bp 1.0 72.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 796-814 bp 1.0 72.0