Hotspot

trnH-GUG-psbA

Genome · 88-268 · 181 bp

Back to Tordylium

Pi 0.0759

MarkerSeek score 76.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 181
variable_sites: 20
pi: 0.0759
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	870-879 bp	1.0	82.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	876-885 bp	1.0	82.2
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	871-880 bp	1.0	82.2
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	872-881 bp	1.0	82.2
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	884-886 bp	0.666667	67.3