primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	88	268	870-879	1.000000	82.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	88	268	876-885	1.000000	82.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	88	268	871-880	1.000000	82.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	88	268	872-881	1.000000	82.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	88	268	884-886	0.666667	67.3
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	GGGGAGGGGTGTTTACCTAT	20	20	45.0	55.0	57.8	58.1	0.0	0.0	0.340559	1331	1530	364-369	1.000000	82.3
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.8	57.8	0.0	18.0	0.464081	1331	1530	315-320	1.000000	82.1
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	GTAAAGACGGTTTTCGGTGC	GGGGAGGGGTGTTTACCTAT	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.402209	1331	1530	365-370	1.000000	81.8
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.8	57.9	0.0	0.0	0.376342	1331	1530	400-405	1.000000	81.6
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.437992	1331	1530	401-406	1.000000	81.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.0	56.5	0.0	13.9	1.591763	4112	4828	841-859	1.000000	72.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	4112	4828	858-876	1.000000	72.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	4112	4828	857-875	1.000000	72.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	4112	4828	797-815	1.000000	72.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	4112	4828	796-814	1.000000	72.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	7536	8105	598-657	1.000000	87.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	20	20	50.0	55.0	57.6	58.1	0.9	0.0	0.478042	7536	8105	584-643	1.000000	84.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.473140	7536	8105	599-658	1.000000	84.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	20	20	55.0	55.0	58.5	58.1	0.0	0.0	0.584056	7536	8105	579-638	1.000000	84.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	GGGGTTACTCGAACGAATCA	20	20	45.0	50.0	58.0	57.3	0.0	0.0	0.698395	7536	8105	597-656	1.000000	82.9
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	8879	9000	202-215	1.000000	71.7
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	8879	9000	205-218	1.000000	71.4
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	8879	9000	208-221	1.000000	70.8
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	8879	9000	211-224	1.000000	70.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	8879	9000	206-219	1.000000	69.2
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCAGCGTAGACATATTGCCA	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.408774	29846	29920	183-183	1.000000	80.2
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACCAGCGTAGACATATTGCC	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.410326	29846	29920	184-184	1.000000	80.2
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CGAATTTACAGTCCGTCCCC	TCTCTTTCAAGGAGGCAACG	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.521328	29846	29920	162-162	1.000000	79.8
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ACGAATTTACAGTCCGTCCC	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.545005	29846	29920	163-163	1.000000	79.6
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GCCAACGAATTTACAGTCCG	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.690519	29846	29920	167-167	1.000000	78.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GAATGGCGAAACGAAAAGGG	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028782	29994	30775	1319-1377	1.000000	88.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GGCGAAACGAAAAGGGAAAG	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052253	29994	30775	1315-1373	1.000000	88.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GAATGGCGAAACGAAAAGGG	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126349	29994	30775	1284-1342	1.000000	88.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GAATGGCGAAACGAAAAGGG	TGGTGATTCCAGAGACGACT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098081	29994	30775	1582-1640	1.000000	87.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GGCGAAACGAAAAGGGAAAG	TGGTGATTCCAGAGACGACT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.121551	29994	30775	1578-1636	1.000000	87.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CTTTTTCACCTTCTGGGCCT	20	20	55.0	50.0	57.9	58.0	0.0	4.5	0.133816	46115	46877	967-1002	1.000000	85.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAACCTGACCTGTTGACCC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.071989	46115	46877	1471-1569	1.000000	85.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAACCTGACCTGTTGACCC	CTTTTTCACCTTCTGGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	4.5	0.068441	46115	46877	1625-1723	1.000000	84.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TAGTCGGAGCCATACCCAAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.101734	46115	46877	1533-1631	1.000000	84.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAGTCGGAGCCATACCCAAT	CTTTTTCACCTTCTGGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	4.5	0.098186	46115	46877	1687-1785	1.000000	84.2
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	CGGAACTAGTTGGTGTGTCC	20	20	50.0	55.0	58.4	58.2	13.2	14.1	0.619320	51976	52162	262-269	1.000000	79.9
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	GGAACTAGTTGGTGTGTCCG	20	20	50.0	55.0	58.4	58.2	13.2	14.1	0.619320	51976	52162	261-268	1.000000	79.9
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.551943	51976	52162	318-325	1.000000	79.6
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.558762	51976	52162	320-327	1.000000	79.5
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TATTGCTTTCATACGGCGGG	AGGCAAATCTAGCTCTCCGA	20	20	50.0	50.0	58.4	58.2	13.2	0.0	0.629540	51976	52162	319-326	1.000000	78.9
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	62385	63019	825-1134	1.000000	74.1
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	62385	63019	812-1121	1.000000	73.7
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	62385	63019	826-1135	1.000000	73.3
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	62385	63019	813-1122	1.000000	73.0
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	62385	63019	830-1139	1.000000	72.4
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	0.0	8.4	0.175650	65371	65514	227-245	1.000000	84.1
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	0.0	8.4	0.232908	65371	65514	226-244	1.000000	83.6
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.252065	65371	65514	219-237	1.000000	83.6
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	6.2	8.4	0.202860	65371	65514	254-272	1.000000	83.3
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	6.2	8.4	0.260118	65371	65514	253-271	1.000000	82.8
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	GTACCGTGAGAGAAGCAAGG	CCTCAGCCTACGGGGTATTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.007701	98340	99511	1244-2380	1.000000	91.9
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	GTACCGTGAGAGAAGCAAGG	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.011083	98340	99511	1418-2554	1.000000	91.9
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	TGAGAGAAGCAAGGAGGTCA	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020826	98340	99511	1238-2374	1.000000	91.8
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	CCGGCCTAGTGAGTCTTTTC	CCTCAGCCTACGGGGTATTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.016910	98340	99511	2012-2470	0.666667	75.6
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	CCGGCCTAGTGAGTCTTTTC	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.020292	98340	99511	2186-2644	0.666667	74.9
ycf1-ndhF_p1	ycf1-ndhF	1	CCCTGCCCACACTAGAAAAA	GTTGGATCCCTCGGAATTCC	20	20	50.0	55.0	57.7	58.0	0.0	15.0	0.317660	110020	110055	1226-1296	1.000000	82.1
ycf1-ndhF_p2	ycf1-ndhF	2	TACGTATCCGCCTAGCTTGT	GTTGGATCCCTCGGAATTCC	20	20	50.0	55.0	58.3	58.0	0.0	15.0	0.347352	110020	110055	1279-1349	1.000000	81.7
ycf1-ndhF_p3	ycf1-ndhF	3	CCCTGCCCACACTAGAAAAA	TGGATCCCTCGGAATTCCTT	20	20	50.0	50.0	57.7	58.1	0.0	22.1	0.393049	110020	110055	1224-1294	1.000000	81.5
ycf1-ndhF_p4	ycf1-ndhF	4	CCCTGCCCACACTAGAAAAA	TTGGATCCCTCGGAATTCCT	20	20	50.0	50.0	57.7	58.1	0.0	22.1	0.393049	110020	110055	1225-1295	1.000000	81.5
ycf1-ndhF_p5	ycf1-ndhF	5	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCG	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.388805	110020	110055	911-911	0.333333	50.2
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCGGGTCGATCAAGTAAC	AGACTCCCCCATCTCCAAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.065727	112303	113246	1655-1741	1.000000	87.5
ndhF-rpl32_p2	ndhF-rpl32	2	CCGTGTGAACCAAAACCCTA	AGACTCCCCCATCTCCAAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.089665	112303	113246	1883-1969	1.000000	86.7
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCGGGTCGATCAAGTAAC	AAAAGACTCCCCCATCTCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.065727	112303	113246	1658-1676	0.666667	71.9
ndhF-rpl32_p4	ndhF-rpl32	4	AAGCGGGTCGATCAAGTAAC	AAAGACTCCCCCATCTCCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.065727	112303	113246	1657-1675	0.666667	71.9
ndhF-rpl32_p5	ndhF-rpl32	5	AAGCGGGTCGATCAAGTAAC	AAGACTCCCCCATCTCCAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.065727	112303	113246	1656-1674	0.666667	71.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.321366	113409	114334	1017-1066	1.000000	85.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.322736	113409	114334	1018-1067	1.000000	84.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCAGCCTTAAAAGCGTTGTC	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.393618	113409	114334	1038-1087	1.000000	84.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAGCCTTAAAAGCGTTGTC	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.393618	113409	114334	1040-1089	1.000000	84.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAGCCTTAAAAGCGTTGTC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.437813	113409	114334	1027-1076	1.000000	84.0
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	CCTCAGCCTACGGGGTATTA	GTACCGTGAGAGAAGCAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.007701	138049	139220	1244-1911	0.666667	71.5
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	CATTGCGGAAAATTCCCCAC	GTACCGTGAGAGAAGCAAGG	20	20	50.0	55.0	58.0	58.0	6.6	0.0	0.011083	138049	139220	1418-2085	0.666667	71.4
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	CCTCAGCCTACGGGGTATTA	TGAGAGAAGCAAGGAGGTCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.020826	138049	139220	1238-1905	0.666667	71.4
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	CCTCAGCCTACGGGGTATTA	CCGGCCTAGTGAGTCTTTTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.016910	138049	139220	2012-2012	0.333333	53.2
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	CATTGCGGAAAATTCCCCAC	CCGGCCTAGTGAGTCTTTTC	20	20	50.0	55.0	58.0	58.0	6.6	0.0	0.020292	138049	139220	2186-2186	0.333333	53.2