Hotspot

petB

LSC · 78465-79926 · 1462 bp

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Pi 0.00114
MarkerSeek score 52.30
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1462
variable_sites
5
pi
0.00114
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TTGATTGGTAGTTCGACCGC ATTGTTCCTCCCCAAGCAAT 50.0 45.0 58.3 57.4 1515-1583 bp 1.0 77.0
petB_p2 TTGATTGGTAGTTCGACCGC TGTTCCTCCCCAAGCAATAAG 50.0 47.6 58.3 57.9 1513-1581 bp 1.0 73.3
petB_p3 TTGATTGGTAGTTCGACCGC TATTGTTCCTCCCCAAGCAA 50.0 45.0 58.3 56.7 1516-1584 bp 1.0 71.7
petB_p4 TTGATTGGTAGTTCGACCGC TGTTCCTCCCCAAGCAATAA 50.0 45.0 58.3 56.7 1513-1581 bp 1.0 71.7
petB_p5 TTGATTGGTAGTTCGACCGC TTGTTCCTCCCCAAGCAATA 50.0 45.0 58.3 56.7 1514-1582 bp 1.0 71.7