Hotspot

ndhB

IRa · 146068-148281 · 2214 bp

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Pi 0.000602
MarkerSeek score 51.40
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
2214
variable_sites
3
pi
0.000602
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 CCTATTCATGGGGATTCCGT GCCCATTCCCATTTTGTTCG 50.0 50.0 56.7 58.0 2408-2410 bp 1.0 74.0
ndhB_p2 TCCTATTCATGGGGATTCCGT GCCCATTCCCATTTTGTTCG 47.6 50.0 58.3 58.0 2409-2411 bp 1.0 73.6
ndhB_p3 CCTATTCATGGGGATTCCGT ATAACCCTAGCCCATTCCCA 50.0 50.0 56.7 57.8 2417-2419 bp 1.0 72.3
ndhB_p4 CCTATTCATGGGGATTCCGT TAACCCTAGCCCATTCCCAT 50.0 50.0 56.7 57.8 2416-2418 bp 1.0 72.3
ndhB_p5 TCCTATTCATGGGGATTCCGT TAACCCTAGCCCATTCCCAT 47.6 50.0 58.3 57.8 2417-2419 bp 1.0 71.9