Hotspot

ndhA

SSC · 124158-126347 · 2190 bp

Pi 0.002

MarkerSeek score 51.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2190
variable_sites: 10
pi: 0.002
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTGGCAAA	ATGGGGGAAGTTGATCGTTG	40.0	50.0	56.0	57.9	2251-2255 bp	1.0	63.6
ndhA_p2	TTGTTTGAGACAAGTGGCAA	ATGGGGGAAGTTGATCGTTG	40.0	50.0	56.0	57.9	2252-2256 bp	1.0	63.6
ndhA_p3	TGTTTGAGACAAGTGGCAAA	GGGGGAAGTTGATCGTTGAA	40.0	50.0	56.0	57.8	2249-2253 bp	1.0	63.1
ndhA_p4	TTGTTTGAGACAAGTGGCAA	GGGGGAAGTTGATCGTTGAA	40.0	50.0	56.0	57.8	2250-2254 bp	1.0	63.1
ndhA_p5	TGAGACAAGTGGCAAATATCGT	ATGGGGGAAGTTGATCGTTG	40.9	50.0	58.1	57.9	2247-2251 bp	1.0	63.1