Hotspot

clpP

LSC · 73483-75584 · 2102 bp

Pi 0.00168

MarkerSeek score 52.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2102
variable_sites: 11
pi: 0.00168
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGTGCATTTGCGTGAAATC	AGTCCATTATTACGCTTCCACA	45.0	40.9	58.0	57.5	2183-2206 bp	1.0	61.0
clpP_p2	TCGTGCATTTGCGTGAAATC	ACGCTTCCACATCAAAGTAAAC	45.0	40.9	58.0	57.5	2172-2195 bp	1.0	61.0
clpP_p3	TCGTGCATTTGCGTGAAATC	ACGCTTCCACATCAAAGTAA	45.0	40.0	58.0	55.3	2172-2195 bp	1.0	59.2
clpP_p4	TCGTGCATTTGCGTGAAATC	TTACGCTTCCACATCAAAGT	45.0	40.0	58.0	55.3	2174-2197 bp	1.0	59.2
clpP_p5	TCGTGCATTTGCGTGAAATC	CGCTTCCACATCAAAGTAAACT	45.0	40.9	58.0	57.3	2171-2194 bp	1.0	58.8