Hotspot

ccsA-ndhD

SSC · 119730-120039 · 310 bp

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Pi 0.012
MarkerSeek score 52.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
310
variable_sites
9
pi
0.012
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AATTCCGCAATTGTGGCTTC AGTGGACAAGGTCGAAGCTA 45.0 50.0 57.6 58.4 394-458 bp 1.0 79.7
ccsA-ndhD_p2 ATTCCGCAATTGTGGCTTCT AGTGGACAAGGTCGAAGCTA 45.0 50.0 58.5 58.4 393-457 bp 1.0 79.0
ccsA-ndhD_p3 TGGAAGGTGTAAATTCCGCA AGTGGACAAGGTCGAAGCTA 45.0 50.0 57.4 58.4 405-469 bp 1.0 78.0
ccsA-ndhD_p4 AATTCCGCAATTGTGGCTTC GTGGACAAGGTCGAAGCTAT 45.0 50.0 57.6 57.3 393-457 bp 1.0 77.3
ccsA-ndhD_p5 AATTCCGCAATTGTGGCTTC ATCAGTGGACAAGGTCGAAG 45.0 50.0 57.6 57.2 397-461 bp 1.0 76.6