Hotspot

ndhF-rpl32

SSC · 116365-117348 · 984 bp

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Pi 0.00828
MarkerSeek score 44.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
984
variable_sites
21
pi
0.00828
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCAGGAATATTGATATGTATGTTCCA TTCAACGCTGCCCAATATCC 34.6 50.0 56.9 58.6 1082-1124 bp 1.0 44.5
ndhF-rpl32_p2 TCCAGGAATATTGATATGTATGTTCCA TTCAACGCTGCCCAATATCC 33.3 50.0 58.1 58.6 1083-1125 bp 1.0 44.5
ndhF-rpl32_p3 CCAGGAATATTGATATGTATGTTCCA AAAAAGCTTTCAACGCTGCC 34.6 45.0 56.9 58.4 1090-1132 bp 1.0 44.4
ndhF-rpl32_p4 TCCAGGAATATTGATATGTATGTTCCA AAAAAGCTTTCAACGCTGCC 33.3 45.0 58.1 58.4 1091-1133 bp 1.0 44.4
ndhF-rpl32_p5 TCCAGGAATATTGATATGTATGTTCC AAAAAGCTTTCAACGCTGCC 34.6 45.0 56.7 58.4 1091-1133 bp 1.0 44.4