Hotspot

rps18-rpl20

LSC · 71857-72114 · 258 bp

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Pi 0.0157
MarkerSeek score 53.40
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
258
variable_sites
14
pi
0.0157
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 TCGCTAGAACTACCGGTCTT TGTATAAGGCGCAGTTGCTT 50.0 45.0 57.9 57.9 397-398 bp 1.0 81.2
rps18-rpl20_p2 TCGCTAGAACTACCGGTCTT AGGCGCAGTTGCTTCTTAAT 50.0 45.0 57.9 58.2 391-392 bp 1.0 80.7
rps18-rpl20_p3 TCGCTAGAACTACCGGTCTT ATAAGGCGCAGTTGCTTCTT 50.0 45.0 57.9 58.2 394-395 bp 1.0 80.7
rps18-rpl20_p4 TCGCTAGAACTACCGGTCTT GGCGCAGTTGCTTCTTAATC 50.0 50.0 57.9 57.8 390-391 bp 1.0 80.7
rps18-rpl20_p5 TCGCTAGAACTACCGGTCTT GCGCAGTTGCTTCTTAATCG 50.0 50.0 57.9 58.2 389-390 bp 1.0 80.5