Hotspot

trnW-CCA-trnP-UGG

LSC · 69973-70147 · 175 bp

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Pi 0.0359
MarkerSeek score 50.80
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
175
variable_sites
22
pi
0.0359
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 255-262 bp 1.0 86.4
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GTGGAAAACAAGACGGGGAT 50.0 50.0 58.0 58.1 340-347 bp 1.0 85.5
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT GGTGCCACGGAAAGATATGT 50.0 50.0 58.0 58.0 678-687 bp 1.0 84.0
trnW-CCA-trnP-UGG_p4 GTGGAATCACGCTCTGTAGG GGTGCCACGGAAAGATATGT 55.0 50.0 58.1 58.0 718-727 bp 1.0 83.5
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT AAGGAAGAGTCAACGCATCC 50.0 50.0 58.0 57.9 764-773 bp 1.0 83.0