Hotspot

trnT-GGU-atpE

LSC · 55276-55534 · 259 bp

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Pi 0.00515
MarkerSeek score 53.00
Primer available yes
Label trnT-GGU-atpE

Feature Metadata

label_name
trnT-GGU-atpE
feature_type
igs
region
LSC
length_bp
259
variable_sites
4
pi
0.00515
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-atpE_p1 CATGTGATGTGATGGGTCCC CTCTCAGACGAGCTAGGACA 55.0 55.0 58.3 58.0 404-428 bp 1.0 82.0
trnT-GGU-atpE_p2 CCATGTGATGTGATGGGTCC CTCTCAGACGAGCTAGGACA 55.0 55.0 58.3 58.0 405-429 bp 1.0 82.0
trnT-GGU-atpE_p3 CATGTGATGTGATGGGTCCC GCTAGGACACGAGTAGAGGT 55.0 55.0 58.3 58.0 393-417 bp 1.0 81.9
trnT-GGU-atpE_p4 CCATGTGATGTGATGGGTCC GCTAGGACACGAGTAGAGGT 55.0 55.0 58.3 58.0 394-418 bp 1.0 81.9
trnT-GGU-atpE_p5 CATGTGATGTGATGGGTCCC CGAGCTAGGACACGAGTAGA 55.0 55.0 58.3 58.1 396-420 bp 1.0 81.7