Hotspot

petN-psbM

LSC · 30371-31495 · 1125 bp

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Pi 0.00276
MarkerSeek score 53.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1125
variable_sites
9
pi
0.00276
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CTTGGGCCGCTTTAATGGTA TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1168-1275 bp 1.0 71.4
petN-psbM_p2 TTGGGCCGCTTTAATGGTAG TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1167-1274 bp 1.0 71.4
petN-psbM_p3 GGCCGCTTTAATGGTAGTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.7 57.6 1164-1271 bp 1.0 70.7
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1119-1226 bp 1.0 70.5
petN-psbM_p5 GGGCCGCTTTAATGGTAGTC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.7 57.6 1165-1272 bp 1.0 67.8