Hotspot

trnS-GCU-trnG-UCC

LSC · 9280-10070 · 791 bp

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Pi 0.00814
MarkerSeek score 51.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
791
variable_sites
21
pi
0.00814
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TAAGCCACTCAGCCATCTCT AACTGAAGGACCCCTTAACT 50.0 45.0 58.1 55.6 695-885 bp 1.0 66.1
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA AACTGAAGGACCCCTTAACT 50.0 45.0 58.2 55.6 762-952 bp 1.0 65.8
trnS-GCU-trnG-UCC_p3 AAGATACCAAGCCATCGACG AACTGAAGGACCCCTTAACT 50.0 45.0 57.8 55.6 785-975 bp 1.0 65.2
trnS-GCU-trnG-UCC_p4 TAAGCCACTCAGCCATCTCT AAACTGAAGGACCCCTTAACT 50.0 42.9 58.1 56.5 696-886 bp 1.0 64.6
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA AAACTGAAGGACCCCTTAACT 50.0 42.9 58.2 56.5 763-953 bp 1.0 64.3