Hotspot

trnK-UUU-rps16

LSC · 4442-5225 · 784 bp

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Pi 0.0185
MarkerSeek score 65.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
784
variable_sites
40
pi
0.0185
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGTACTTAAAAGCCGAG TCAAAGAAGGCAGGGGTTTT 50.0 45.0 58.0 57.5 864-1086 bp 1.0 83.2
trnK-UUU-rps16_p2 AGCCGTACTTAAAAGCCGAG TTCAAAGAAGGCAGGGGTTT 50.0 45.0 58.0 57.5 865-1087 bp 1.0 83.2
trnK-UUU-rps16_p3 AGCCGTACTTAAAAGCCGAG TTTCAAAGAAGGCAGGGGTT 50.0 45.0 58.0 57.5 866-1088 bp 1.0 83.2
trnK-UUU-rps16_p4 AGCCGTACTTAAAAGCCGAG CAACCTACAGGAACCGTTCA 50.0 50.0 58.0 57.5 891-1113 bp 1.0 82.4
trnK-UUU-rps16_p5 AGCCGTACTTAAAAGCCGAG ATTTCAAAGAAGGCAGGGGT 50.0 45.0 58.0 57.3 867-1089 bp 1.0 81.3