Hotspot

ndhG-ndhI

SSC · 121217-121658 · 442 bp

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Pi 0.0447

MarkerSeek score 70.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 442
variable_sites: 55
pi: 0.0447
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1296-1458 bp	1.0	88.5
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1295-1457 bp	1.0	88.5
ndhG-ndhI_p3	GCAATCGCACCTATTAACGC	TGCAAGGTACATCGGTCAAG	50.0	50.0	58.0	57.9	1280-1442 bp	1.0	88.1
ndhG-ndhI_p4	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	50.0	45.0	58.0	57.9	915-1077 bp	0.928571	86.2
ndhG-ndhI_p5	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	50.0	45.0	58.0	57.9	914-1076 bp	0.928571	86.1