Pi
0.0231
MarkerSeek score
74.30
Primer available
yes
Label
ndhF-rpl32
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhF-rpl32_p1 | AGGAAAGGTATTATCCACGCA | TAAGGCTGCCCAGTATCCTT | 42.9 | 50.0 | 56.8 | 58.1 | 1149-1401 bp | 1.0 | 72.8 |
| ndhF-rpl32_p2 | AGGAAAGGTATTATCCACGCA | TTAAGGCTGCCCAGTATCCT | 42.9 | 50.0 | 56.8 | 58.1 | 1150-1402 bp | 1.0 | 72.8 |
| ndhF-rpl32_p3 | AGGAAAGGTATTATCCACGCA | GCTTTTAAGGCTGCCCAGTA | 42.9 | 50.0 | 56.8 | 58.2 | 1154-1406 bp | 1.0 | 72.3 |
| ndhF-rpl32_p4 | AGGAAAGGTATTATCCACGCA | GACAACGCTTTTAAGGCTGC | 42.9 | 50.0 | 56.8 | 58.3 | 1160-1412 bp | 1.0 | 71.1 |
| ndhF-rpl32_p5 | AGGAAAGGTATTATCCACGCA | TGCCCAGTATCCTTTCCCTT | 42.9 | 50.0 | 56.8 | 58.3 | 1143-1395 bp | 1.0 | 71.1 |