Hotspot

trnN-GUU-ndhF

IRb · 110105-112331 · 2227 bp

Back to Tongoloa

Pi 0.00729

MarkerSeek score 52.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2227
variable_sites: 70
pi: 0.00729
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	GGGGGAATAAGAGGATTGGC	55.0	55.0	58.0	57.7	2182-2562 bp	1.0	84.8
trnN-GUU-ndhF_p2	ATCTCCCCAGGTAGGATTCG	ACGTTGTTTGTTGGATCCCT	55.0	45.0	58.0	57.6	2539-2919 bp	1.0	84.5
trnN-GUU-ndhF_p3	TCAAAGCGAGCCCTTTTCTT	GGGGGAATAAGAGGATTGGC	45.0	55.0	58.0	57.7	2235-2615 bp	0.857143	79.0
trnN-GUU-ndhF_p4	GAAACAAAGACGGGGAAGGT	GGGGGAATAAGAGGATTGGC	50.0	55.0	58.0	57.7	2273-2653 bp	0.857143	78.9
trnN-GUU-ndhF_p5	AGGATTATCCCGGACCTACG	GGGGGAATAAGAGGATTGGC	55.0	55.0	58.1	57.7	2366-2746 bp	0.857143	78.4