Hotspot

psaJ-rpl33

LSC · 68993-69444 · 452 bp

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Pi 0.0123

MarkerSeek score 54.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 29
pi: 0.0123
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	GGTCGACTCGGTTCTTTCAA	45.0	50.0	58.0	57.9	1170-1253 bp	1.0	88.4
psaJ-rpl33_p2	CATATCTTTCCGTGGCACCA	GGTCGACTCGGTTCTTTCAA	50.0	50.0	58.0	57.9	1248-1331 bp	1.0	88.0
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	GGTCGACTCGGTTCTTTCAA	50.0	50.0	58.0	57.9	1249-1332 bp	1.0	88.0
psaJ-rpl33_p4	TTTCGGTAATGCTCGGGAAG	GGTCGACTCGGTTCTTTCAA	50.0	50.0	58.0	57.9	1465-1538 bp	0.857143	82.0
psaJ-rpl33_p5	TTTCGGTAATGCTCGGGAAG	TTAGGCGTGTTGTGACGATT	50.0	45.0	58.0	57.8	899-932 bp	0.857143	78.3