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rps4-trnT-UGU

LSC · 48075-48496 · 422 bp

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Pi 0.0382

MarkerSeek score 68.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 60
pi: 0.0382
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GGTTTAGGTCTGACGCGAAT	ACGGTCGATTCTTGAGCTTC	50.0	50.0	58.0	58.0	1581 bp	0.071429	42.8
rps4-trnT-UGU_p2	GGTTTAGGTCTGACGCGAAT	GAACGGTCGATTCTTGAGCT	50.0	50.0	58.0	58.0	1583 bp	0.071429	42.8
rps4-trnT-UGU_p3	AAAACCTGACCTGTTGACCC	ACGGTCGATTCTTGAGCTTC	50.0	50.0	57.9	58.0	1220 bp	0.071429	42.3
rps4-trnT-UGU_p4	AAAACCTGACCTGTTGACCC	GAACGGTCGATTCTTGAGCT	50.0	50.0	57.9	58.0	1222 bp	0.071429	42.3
rps4-trnT-UGU_p5	GTCCGAACCCCTGTGATTTT	ACGGTCGATTCTTGAGCTTC	50.0	50.0	58.1	58.0	1620 bp	0.071429	42.1