Hotspot

trnE-UUC-trnT-GGU

LSC · 31985-32928 · 944 bp

Back to Tongoloa

Pi 0.0258

MarkerSeek score 68.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 101
pi: 0.0258
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CAAGAAGAGACCATTCCGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	469-1224 bp	0.714286	78.4
trnE-UUC-trnT-GGU_p2	CCAAGAAGAGACCATTCCGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	470-1225 bp	0.714286	78.4
trnE-UUC-trnT-GGU_p3	CCCAAGAAGAGACCATTCCG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	471-1226 bp	0.714286	78.4
trnE-UUC-trnT-GGU_p4	CAAGAAGAGACCATTCCGGG	AAAGTCAAAGCCCCTTACCG	55.0	50.0	58.0	58.1	494-1249 bp	0.714286	77.6
trnE-UUC-trnT-GGU_p5	CCAAGAAGAGACCATTCCGG	AAAGTCAAAGCCCCTTACCG	55.0	50.0	58.0	58.1	495-1250 bp	0.714286	77.6