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psbM-trnD-GUC

LSC · 30855-31562 · 708 bp

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Pi 0.0115

MarkerSeek score 68.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 39
pi: 0.0115
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.2	58.5	822-846 bp	1.0	68.5
psbM-trnD-GUC_p2	AAGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.9	58.5	823-847 bp	1.0	65.9
psbM-trnD-GUC_p3	AGCAGAAAAGCAGTAGGAACT	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.2	57.0	823-847 bp	1.0	64.3
psbM-trnD-GUC_p4	AGCAGAAAAGCAGTAGGAAC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	822-846 bp	1.0	64.1
psbM-trnD-GUC_p5	GCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	821-845 bp	1.0	64.1