Hotspot

rpoB-trnC-GCA

LSC · 27924-29163 · 1240 bp

Back to Tongoloa

Pi 0.0127

MarkerSeek score 67.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1240
variable_sites: 74
pi: 0.0127
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1135-1335 bp	1.0	86.7
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1175-1375 bp	1.0	86.5
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCTCGGA	TTATTGATCAGGCGACACCC	50.0	50.0	57.8	57.7	1193-1393 bp	1.0	84.5
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	AAGGGATTGGATTTCGAGCC	50.0	50.0	57.8	57.9	1366 bp	0.071429	41.0
rpoB-trnC-GCA_p5	TCCCTCATTTCCATCTCGGA	GGGATTGGATTTCGAGCCTT	50.0	50.0	57.8	57.9	1364 bp	0.071429	41.0