Hotspot

trnH-GUG-psbA

LSC · 254-582 · 329 bp

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Pi 0.0622
MarkerSeek score 82.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
329
variable_sites
67
pi
0.0622
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 438-489 bp 1.0 89.7
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 437-488 bp 1.0 89.7
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 439-490 bp 1.0 89.7
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG TGGAAGTTATGCACGAACGT 55.0 45.0 58.1 57.8 424-475 bp 0.928571 86.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TGCACGAACGTAATGCTCAT 55.0 45.0 58.1 58.3 415-466 bp 0.928571 85.9