Hotspot

ndhD

SSC · 118352-119856 · 1505 bp

Back to Tofieldia

Pi 0.00776

MarkerSeek score 50.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1505
variable_sites: 17
pi: 0.00776
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTCTCTGGAGCTGTATGGG	AGATGTGAATCCGCTTGTCC	55.0	50.0	57.9	58.0	2150-2156 bp	1.0	81.0
ndhD_p2	TCTCTCTGGAGCTGTATGGG	CTTTACCGACAAAAACCCGC	55.0	50.0	57.9	58.0	2018-2028 bp	1.0	81.0
ndhD_p3	TCTCTCTGGAGCTGTATGGG	TATGGCATGAGACAACTCGC	55.0	50.0	57.9	58.1	2101-2107 bp	1.0	80.8
ndhD_p4	GTTGTGCGGGTTTTTGAGAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1834-1836 bp	1.0	80.6
ndhD_p5	GGCTCTCAAAAACCCGAGAT	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1782-1784 bp	1.0	80.0