Hotspot

clpP1

LSC · 72255-74257 · 2003 bp

Back to Tofieldia

Pi 0.0127

MarkerSeek score 56.20

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2003
variable_sites: 38
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	CGCGGAAAATTCGTTCGAAA	CCATTGTTACGTTTCCACATCA	45.0	40.9	58.1	57.2	2111-2157 bp	1.0	59.4
clpP1_p2	AATCGCGGAAAATTCGTTCG	CCATTGTTACGTTTCCACATCA	45.0	40.9	57.8	57.2	2114-2160 bp	1.0	58.6
clpP1_p3	CGCGGAAAATTCGTTCGAAA	CCATTGTTACGTTTCCACATCAA	45.0	39.1	58.1	57.9	2111-2157 bp	1.0	56.5
clpP1_p4	AATCGCGGAAAATTCGTTCG	CCATTGTTACGTTTCCACATCAA	45.0	39.1	57.8	57.9	2114-2160 bp	1.0	55.8
clpP1_p5	ATCGCGGAAAATTCGTTCGA	CCATTGTTACGTTTCCACATCA	45.0	40.9	58.6	57.2	2113-2159 bp	1.0	54.7