Hotspot

rpl32-trnL-UAG

SSC · 116251-117010 · 760 bp

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Pi 0.0217

MarkerSeek score 65.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 24
pi: 0.0217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGTTTCCACAGGACATTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	838-864 bp	1.0	84.4
rpl32-trnL-UAG_p2	CGGTTTCCACAGGACATTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.1	57.8	831-857 bp	1.0	83.9
rpl32-trnL-UAG_p3	TCGGTTTCCACAGGACATTC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	839-865 bp	1.0	83.8
rpl32-trnL-UAG_p4	CGGTTTCCACAGGACATTCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.1	57.8	837-863 bp	1.0	83.8
rpl32-trnL-UAG_p5	CGGTTTCCACAGGACATTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	836-862 bp	1.0	83.8