Hotspot

ndhF-rpl32

SSC · 115500-116076 · 577 bp

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Pi 0.0277

MarkerSeek score 58.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 577
variable_sites: 24
pi: 0.0277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTAACGGGAAGTGGAAGGA	AAAGCTTTTAACGCAGCCAA	50.0	40.0	58.0	57.1	729-959 bp	1.0	79.2
ndhF-rpl32_p2	AGTAACGGGAAGTGGAAGGA	AAGCTTTTAACGCAGCCAAA	50.0	40.0	58.0	57.1	728-958 bp	1.0	79.2
ndhF-rpl32_p3	AGTAACGGGAAGTGGAAGGA	AAAAGCTTTTAACGCAGCCA	50.0	40.0	58.0	57.1	730-960 bp	1.0	79.1
ndhF-rpl32_p4	AGTAACGGGAAGTGGAAGGA	AGCTTTTAACGCAGCCAAAT	50.0	40.0	58.0	56.9	727-957 bp	1.0	77.3
ndhF-rpl32_p5	AACGGGAAGTGGAAGGAAAG	AAGCTTTTAACGCAGCCAAA	50.0	40.0	57.7	57.1	725-955 bp	1.0	77.1