Hotspot

rpl22-rps19

LSC · 86475-86546 · 72 bp

Back to Tofieldia

Pi 0.0463

MarkerSeek score 72.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 5
pi: 0.0463
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACTTGGACTTCTGTACCCGA	TGACTTTCGGTAGACATGCG	50.0	50.0	58.0	58.0	169-177 bp	1.0	82.2
rpl22-rps19_p2	TGGACTTCTGTACCCGATGT	TGACTTTCGGTAGACATGCG	50.0	50.0	58.1	58.0	166-174 bp	1.0	81.8
rpl22-rps19_p3	ACTTGGACTTCTGTACCCGA	GACTTTCGGTAGACATGCGA	50.0	50.0	58.0	57.7	168-176 bp	1.0	80.1
rpl22-rps19_p4	ACTTGGACTTCTGTACCCGA	ACTTTCGGTAGACATGCGAG	50.0	50.0	58.0	57.7	167-175 bp	1.0	80.1
rpl22-rps19_p5	TGGACTTCTGTACCCGATGT	GACTTTCGGTAGACATGCGA	50.0	50.0	58.1	57.7	165-173 bp	1.0	79.7