Hotspot

rpl36-infA

LSC · 82171-82279 · 109 bp

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Pi 0.275

MarkerSeek score 66.20

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 45
pi: 0.275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ATAATTCGTCTGCGCCTACG	TGATTCAACCAGGGGACGTA	50.0	50.0	58.2	58.4	238-240 bp	1.0	83.3
rpl36-infA_p2	CGCCTACGGATCAGTCTACA	TGATTCAACCAGGGGACGTA	55.0	50.0	58.7	58.4	226-228 bp	1.0	79.6
rpl36-infA_p3	ATAATTCGTCTGCGCCTACG	TATGATTCAACCAGGGGACG	50.0	50.0	58.2	57.0	240-242 bp	1.0	78.1
rpl36-infA_p4	ATAATTCGTCTGCGCCTACG	GATTCAACCAGGGGACGTAT	50.0	50.0	58.2	57.0	237-239 bp	1.0	78.1
rpl36-infA_p5	ATAATTCGTCTGCGCCTACG	ATGATTCAACCAGGGGACGT	50.0	50.0	58.2	59.0	239-241 bp	1.0	78.1