Hotspot

psbT-pbf1

LSC · 76524-76587 · 64 bp

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Pi 0.0937

MarkerSeek score 86.90

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 9
pi: 0.0937
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	ACCACCTAAGGTTCCGACTA	TCGCCATCTCCATATCTGGT	50.0	50.0	57.4	58.0	211 bp	1.0	78.4
psbT-pbf1_p2	ACCACCTAAGGTTCCGACTA	CCCTAGTCGCCATCTCCATA	50.0	55.0	57.4	58.1	217 bp	1.0	77.8
psbT-pbf1_p3	ACCACCTAAGGTTCCGACTA	GATCCATTCGAGGAACACGG	50.0	55.0	57.4	58.4	125 bp	1.0	77.5
psbT-pbf1_p4	ACCACCTAAGGTTCCGACTA	GGGTACGCCTTATATACCGC	50.0	55.0	57.4	57.7	173 bp	1.0	77.0
psbT-pbf1_p5	ACCACCTAAGGTTCCGACTA	GTCGCCATCTCCATATCTGG	50.0	55.0	57.4	57.6	212 bp	1.0	75.5