Hotspot

accD-psaI

LSC · 61027-62120 · 1094 bp

Back to Tofieldia

Pi 0.026

MarkerSeek score 75.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 36
pi: 0.026
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCGGCTGAGTATTCATTCCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	982-1284 bp	1.0	85.9
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	973-1275 bp	1.0	85.8
accD-psaI_p3	GCGGCTGAGTATTCATTCCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	988-1290 bp	1.0	85.3
accD-psaI_p4	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	897-1199 bp	1.0	84.7
accD-psaI_p5	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	896-1198 bp	1.0	84.7