Hotspot

rbcL-accD

LSC · 58677-59670 · 994 bp

Back to Tofieldia

Pi 0.0272
MarkerSeek score 67.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
994
variable_sites
34
pi
0.0272
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TCGAACCAGTGGATAAGATAGA GGAGTCTTCACTTCCATCGG 40.9 55.0 55.8 57.7 966-1171 bp 1.0 50.7
rbcL-accD_p2 TTCGAACCAGTGGATAAGAT GGAGTCTTCACTTCCATCGG 40.0 55.0 53.8 57.7 967-1172 bp 1.0 50.2
rbcL-accD_p3 TTCGAACCAGTGGATAAGATAGA GGAGTCTTCACTTCCATCGG 39.1 55.0 56.6 57.7 967-1172 bp 1.0 48.5
rbcL-accD_p4 TCGAACCAGTGGATAAGATAGA TCGAATTGAACCACCACCTT 40.9 45.0 55.8 57.4 871-1076 bp 1.0 48.2
rbcL-accD_p5 TTCGAACCAGTGGATAAGAT TCGAATTGAACCACCACCTT 40.0 45.0 53.8 57.4 872-1077 bp 1.0 47.6