Hotspot

trnT-GGU-psbD

LSC · 33057-34179 · 1123 bp

Back to Tofieldia

Pi 0.011

MarkerSeek score 61.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 18
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1224-1277 bp	1.0	81.8
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1208-1261 bp	1.0	81.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GTCCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1238-1291 bp	1.0	81.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1227-1280 bp	1.0	81.6
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1292-1296 bp	0.666667	64.0