Hotspot

atpH-atpI

LSC · 14613-15632 · 1020 bp

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Pi 0.00734

MarkerSeek score 65.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 11
pi: 0.00734
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1071-1108 bp	1.0	80.4
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.1	58.5	1159-1196 bp	1.0	80.1
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	1044-1081 bp	1.0	79.8
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1127-1164 bp	1.0	79.7
atpH-atpI_p5	CGCAATACCTTCTACGGCTT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.1	57.4	1132-1169 bp	1.0	79.6