Hotspot

trnH-GUG-psbA

LSC · 76-415 · 340 bp

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Pi 0.0338

MarkerSeek score 63.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 17
pi: 0.0338
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TAGTTGACAGTCAGGGTCGT	55.0	50.0	58.1	58.0	388-521 bp	1.0	86.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TAGTTGACAGTCAGGGTCGT	50.0	50.0	57.9	58.0	402-535 bp	1.0	86.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TAGTTGACAGTCAGGGTCGT	50.0	50.0	57.9	58.0	401-534 bp	1.0	86.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TAGTTGACAGTCAGGGTCGT	50.0	50.0	57.9	58.0	403-536 bp	1.0	86.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TGCATGGTTCCTTGGTAACC	55.0	50.0	58.1	58.1	664-797 bp	1.0	84.4