Hotspot

ndhK

LSC · 49366-50073 · 708 bp

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Pi 0.0383

MarkerSeek score 72.30

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 708
variable_sites: 39
pi: 0.0383
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GCACACTGGGAGCGTAAATA	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.0	58.0	1023-1036 bp	1.0	86.0
ndhK_p2	TCTTTACGTAAAGGCCAGCC	AAAAGGAGCGTTGGAATGGT	50.0	45.0	57.9	58.0	1286-1303 bp	1.0	84.8
ndhK_p3	GCCAGCCTATCCAACTTTCA	AAAAGGAGCGTTGGAATGGT	50.0	45.0	57.9	58.0	1273-1290 bp	1.0	84.6
ndhK_p4	GGGGACGCTCATTGAATGAT	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.0	58.0	1404-1421 bp	0.666667	70.8
ndhK_p5	GAAGGGGACGCTCATTGAAT	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.0	58.0	1407-1424 bp	0.666667	70.6