Hotspot

ndhF-rpl32

SSC · 112823-113731 · 909 bp

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Pi 0.0393

MarkerSeek score 70.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 909
variable_sites: 48
pi: 0.0393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACCACATAGCTA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1211-1344 bp	0.666667	70.6
ndhF-rpl32_p2	AGATTGGCCGAAAACCACAT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1216-1349 bp	0.666667	70.2
ndhF-rpl32_p3	TGGGAGTGGAAAAAGGGGTA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.2	58.0	1094 bp	0.333333	51.7
ndhF-rpl32_p4	CGGATTTAGCAATTGCACCG	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.2	58.0	1767 bp	0.333333	51.6
ndhF-rpl32_p5	GGCCGAAAACCACATAGCTA	TCTCAGTCGCGGGGTATAAA	50.0	50.0	58.0	58.2	1658 bp	0.333333	51.2