Hotspot

trnR-ACG-trnN-GUU

IRb · 108677-109243 · 567 bp

Back to Tipularia

Pi 0.0329

MarkerSeek score 71.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 567
variable_sites: 24
pi: 0.0329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	495-716 bp	1.0	85.3
trnR-ACG-trnN-GUU_p2	TTTGAAGGTACTCCCGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	548-769 bp	1.0	84.6
trnR-ACG-trnN-GUU_p3	TTTTGAAGGTACTCCCGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	549-770 bp	1.0	84.6
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	459-680 bp	1.0	84.3
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	446-667 bp	1.0	84.3