Hotspot

accD-psaI

LSC · 58614-59459 · 846 bp

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Pi 0.0282

MarkerSeek score 71.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 34
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AAGAAACCATTGCGATTGCC	50.0	45.0	58.0	57.6	910-952 bp	1.0	83.6
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	50.0	55.0	58.0	58.4	898-940 bp	1.0	83.2
accD-psaI_p3	CACGGTTTCCTTCCCTTGAA	CGGAAAGACTAGGCCCACTA	50.0	55.0	58.0	58.5	891-933 bp	1.0	82.6
accD-psaI_p4	TCAGCTACACGGTTTCCTTC	AAGAAACCATTGCGATTGCC	50.0	45.0	57.5	57.6	917-959 bp	1.0	80.1
accD-psaI_p5	TCAGCTACACGGTTTCCTTC	CGATTGCCGGAAAGACTAGG	50.0	55.0	57.5	58.4	905-947 bp	1.0	79.8