Hotspot

trnS-GCU-trnG-UCC

LSC · 8272-9200 · 929 bp

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Pi 0.0382
MarkerSeek score 69.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
929
variable_sites
43
pi
0.0382
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.84
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AGTCCACTCAGCCATCTCTC TACCACTAAACTATACCCGC 55.0 45.0 58.5 54.0 872-970 bp 1.0 51.5
trnS-GCU-trnG-UCC_p2 AGTCCACTCAGCCATCTCTC TTACCACTAAACTATACCCGC 55.0 42.9 58.5 54.9 873-971 bp 1.0 50.4
trnS-GCU-trnG-UCC_p3 TAGTCCACTCAGCCATCTCT TACCACTAAACTATACCCGC 50.0 45.0 57.2 54.0 873-971 bp 1.0 48.9
trnS-GCU-trnG-UCC_p4 CACTTTAGTCCACTCAGCCA TACCACTAAACTATACCCGC 50.0 45.0 57.2 54.0 878-976 bp 1.0 48.9
trnS-GCU-trnG-UCC_p5 ACACTTTAGTCCACTCAGCC TACCACTAAACTATACCCGC 50.0 45.0 57.2 54.0 879-977 bp 1.0 48.9