primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TTTTACTTTGGTATGGGTTGA	TGATCATATCTAAACTTCACTTCA	21	24	33.3	29.2	52.9	53.5	0.0	0.0	14.543189	1464	4299	2890-2890	0.333333	13.3
trnK-UUU_p2	trnK-UUU	2	TTTTTACTTTGGTATGGGTTGA	TGATCATATCTAAACTTCACTTCA	22	24	31.8	29.2	53.8	53.5	0.0	0.0	14.651974	1464	4299	2891-2891	0.333333	13.3
trnK-UUU_p3	trnK-UUU	3	TTTTACTTTGGTATGGGTTGA	TCTAAACTTCACTTCATATTGATCA	21	25	33.3	28.0	52.9	54.3	0.0	0.0	14.786622	1464	4299	2882-2882	0.333333	13.3
trnK-UUU_p4	trnK-UUU	4	TTTTACTTTGGTATGGGTTGA	TTGATCATATCTAAACTTCACTTCA	21	25	33.3	28.0	52.9	54.3	0.0	0.0	14.786622	1464	4299	2891-2891	0.333333	13.3
trnK-UUU_p5	trnK-UUU	5	TTTTTACTTTGGTATGGGTTGA	TCTAAACTTCACTTCATATTGATCA	22	25	31.8	28.0	53.8	54.3	0.0	0.0	14.895408	1464	4299	2883-2883	0.333333	13.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	AGTCCACTCAGCCATCTCTC	TACCACTAAACTATACCCGC	20	20	55.0	45.0	58.5	54.0	0.0	0.0	4.527038	8272	9200	872-970	1.000000	51.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGTCCACTCAGCCATCTCTC	TTACCACTAAACTATACCCGC	20	21	55.0	42.9	58.5	54.9	0.0	0.0	4.663298	8272	9200	873-971	1.000000	50.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TAGTCCACTCAGCCATCTCT	TACCACTAAACTATACCCGC	20	20	50.0	45.0	57.2	54.0	0.0	0.0	4.847712	8272	9200	873-971	1.000000	48.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CACTTTAGTCCACTCAGCCA	TACCACTAAACTATACCCGC	20	20	50.0	45.0	57.2	54.0	0.0	0.0	4.850562	8272	9200	878-976	1.000000	48.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACACTTTAGTCCACTCAGCC	TACCACTAAACTATACCCGC	20	20	50.0	45.0	57.2	54.0	0.0	0.0	4.852518	8272	9200	879-977	1.000000	48.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAAAAAGAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.0	0.0	0.063299	46455	47025	661-672	1.000000	86.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46455	47025	610-621	1.000000	85.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AGAAAAAGAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.0	0.0	0.128108	46455	47025	669-680	1.000000	85.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46455	47025	618-629	1.000000	85.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46455	47025	642-653	1.000000	84.9
ndhK_p1	ndhK	1	GCACACTGGGAGCGTAAATA	AAAAGGAGCGTTGGAATGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023166	49366	50073	1023-1036	1.000000	86.0
ndhK_p2	ndhK	2	TCTTTACGTAAAGGCCAGCC	AAAAGGAGCGTTGGAATGGT	20	20	50.0	45.0	57.9	58.0	35.6	0.0	0.108161	49366	50073	1286-1303	1.000000	84.8
ndhK_p3	ndhK	3	GCCAGCCTATCCAACTTTCA	AAAAGGAGCGTTGGAATGGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.133394	49366	50073	1273-1290	1.000000	84.6
ndhK_p4	ndhK	4	GGGGACGCTCATTGAATGAT	AAAAGGAGCGTTGGAATGGT	20	20	50.0	45.0	58.0	58.0	8.5	0.0	0.031752	49366	50073	1404-1421	0.666667	70.8
ndhK_p5	ndhK	5	GAAGGGGACGCTCATTGAAT	AAAAGGAGCGTTGGAATGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048746	49366	50073	1407-1424	0.666667	70.6
accD-psaI_p1	accD-psaI	1	CACGGTTTCCTTCCCTTGAA	AAGAAACCATTGCGATTGCC	20	20	50.0	45.0	58.0	57.6	0.0	1.5	0.392995	58614	59459	910-952	1.000000	83.6
accD-psaI_p2	accD-psaI	2	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	20	20	50.0	55.0	58.0	58.4	0.0	5.0	0.452422	58614	59459	898-940	1.000000	83.2
accD-psaI_p3	accD-psaI	3	CACGGTTTCCTTCCCTTGAA	CGGAAAGACTAGGCCCACTA	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.542469	58614	59459	891-933	1.000000	82.6
accD-psaI_p4	accD-psaI	4	TCAGCTACACGGTTTCCTTC	AAGAAACCATTGCGATTGCC	20	20	50.0	45.0	57.5	57.6	0.0	1.5	0.819264	58614	59459	917-959	1.000000	80.1
accD-psaI_p5	accD-psaI	5	TCAGCTACACGGTTTCCTTC	CGATTGCCGGAAAGACTAGG	20	20	50.0	55.0	57.5	58.4	0.0	5.0	0.878691	58614	59459	905-947	1.000000	79.8
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	TCGAAGAACATGGGGACTAG	21	20	47.6	50.0	57.6	56.4	0.0	0.0	3.037025	74421	74484	122-123	1.000000	60.3
psbT-psbN_p2	psbT-psbN	2	GCGATCGAATCTATGGAAGCA	TCGAAGAACATGGGGACTAG	21	20	47.6	50.0	58.3	56.4	21.6	0.0	2.963566	74421	74484	203-204	1.000000	59.0
psbT-psbN_p3	psbT-psbN	3	CGAGAACCACCTAAAGTTCCA	TTCGAAGAACATGGGGACTAG	21	21	47.6	47.6	57.6	57.1	0.0	0.0	3.283881	74421	74484	123-124	1.000000	58.3
psbT-psbN_p4	psbT-psbN	4	GCGATCGAATCTATGGAAGC	TCGAAGAACATGGGGACTAG	20	20	50.0	50.0	56.6	56.4	21.6	0.0	3.071914	74421	74484	203-204	1.000000	58.2
psbT-psbN_p5	psbT-psbN	5	GCGATCGAATCTATGGAAGCA	TTCGAAGAACATGGGGACTAG	21	21	47.6	47.6	58.3	57.1	21.6	0.0	3.210422	74421	74484	204-205	1.000000	57.1
rps11-rpl36_p1	rps11-rpl36	1	ATTTTTGCTATAGGTTTTGTCA	GGCGAGGGCGAATTATAGTC	22	20	27.3	55.0	52.3	58.3	1.0	0.0	8.029800	79725	79894	252-261	1.000000	48.7
rps11-rpl36_p2	rps11-rpl36	2	TTTTTGCTATAGGTTTTGTCAT	GGCGAGGGCGAATTATAGTC	22	20	27.3	55.0	52.3	58.3	1.0	0.0	8.029800	79725	79894	251-260	1.000000	48.7
rps11-rpl36_p3	rps11-rpl36	3	TTTTTGCTATAGGTTTTGTCAT	GCGAGGGCGAATTATAGTCA	22	20	27.3	50.0	52.3	57.6	1.0	0.0	8.174875	79725	79894	250-259	1.000000	48.7
rps11-rpl36_p4	rps11-rpl36	4	ATTTTTGCTATAGGTTTTGTCA	ATGTCGCTTGATTCGTAGGC	22	20	27.3	50.0	52.3	58.4	1.0	0.0	8.169088	79725	79894	269-278	1.000000	48.4
rps11-rpl36_p5	rps11-rpl36	5	TTTTTGCTATAGGTTTTGTCAT	ATGTCGCTTGATTCGTAGGC	22	20	27.3	50.0	52.3	58.4	1.0	0.0	8.169088	79725	79894	268-277	1.000000	48.4
rps3-rpl22_p1	rps3-rpl22	1	CGATGTTGAGTCTCCGGTTT	TTATCATTCATTGGCGGGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.086330	83959	84019	793-798	1.000000	82.2
rps3-rpl22_p2	rps3-rpl22	2	CGATGTTGAGTCTCCGGTTT	TTCGCGGACGTTCTTATGAG	20	20	50.0	50.0	57.9	58.1	0.0	6.1	0.176142	83959	84019	670-675	1.000000	81.8
rps3-rpl22_p3	rps3-rpl22	3	ACGTGCAATCTCTTTTCCGT	TTATCATTCATTGGCGGGGG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.127091	83959	84019	981-986	1.000000	81.4
rps3-rpl22_p4	rps3-rpl22	4	TCGACACGTGCAATCTCTTT	TTATCATTCATTGGCGGGGG	20	20	45.0	50.0	57.9	58.0	17.2	0.0	0.154960	83959	84019	986-991	1.000000	81.3
rps3-rpl22_p5	rps3-rpl22	5	TTCGACACGTGCAATCTCTT	TTATCATTCATTGGCGGGGG	20	20	45.0	50.0	57.9	58.0	17.2	0.0	0.154960	83959	84019	987-992	1.000000	81.3
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	85057	85099	179-179	1.000000	80.5
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	85057	85099	178-178	1.000000	80.5
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	85057	85099	180-180	1.000000	80.5
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	85057	85099	193-193	1.000000	80.4
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.1	58.2	0.2	35.0	0.281592	85057	85099	207-207	1.000000	79.3
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	108677	109243	495-716	1.000000	85.3
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	TTTGAAGGTACTCCCGGGAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.3	58.0	26.7	0.0	0.270214	108677	109243	548-769	1.000000	84.6
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	TTTTGAAGGTACTCCCGGGA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.3	58.0	25.1	0.0	0.270214	108677	109243	549-770	1.000000	84.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	20	20	55.0	50.0	57.8	57.8	18.9	15.9	0.366304	108677	109243	459-680	1.000000	84.3
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	108677	109243	446-667	1.000000	84.3
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	AGATCAGAATTGGTGAATCGGA	20	22	50.0	40.9	58.0	57.2	15.2	0.0	2.805323	110603	112822	2441-2441	0.333333	30.9
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	AGATCAGAATTGGTGAATCGGA	20	22	50.0	40.9	58.0	57.2	15.2	0.0	2.807909	110603	112822	2433-2433	0.333333	30.9
ndhF_p3	ndhF	3	AGAGGGATCCACCGAAGAAA	AGATCAGAATTGGTGAATCGGA	20	22	50.0	40.9	58.0	57.2	15.2	0.0	2.807909	110603	112822	2432-2432	0.333333	30.9
ndhF_p4	ndhF	4	ACAGGAACAAGAGGGATCCA	CAGAATTGGTGAATCGGAAACA	20	22	50.0	40.9	58.0	57.2	15.2	0.0	2.809471	110603	112822	2437-2437	0.333333	30.9
ndhF_p5	ndhF	5	AGAGGGATCCACCGAAGAAA	CAGAATTGGTGAATCGGAAACA	20	22	50.0	40.9	58.0	57.2	15.2	0.0	2.812057	110603	112822	2428-2428	0.333333	30.8
ndhF-rpl32_p1	ndhF-rpl32	1	GGCCGAAAACCACATAGCTA	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.070887	112823	113731	1211-1344	0.666667	70.6
ndhF-rpl32_p2	ndhF-rpl32	2	AGATTGGCCGAAAACCACAT	CTTTTTGACTGTCCGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.114851	112823	113731	1216-1349	0.666667	70.2
ndhF-rpl32_p3	ndhF-rpl32	3	TGGGAGTGGAAAAAGGGGTA	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.202311	112823	113731	1094-1094	0.333333	51.7
ndhF-rpl32_p4	ndhF-rpl32	4	CGGATTTAGCAATTGCACCG	CTTTTTGACTGTCCGGTGGA	20	20	50.0	50.0	58.2	58.0	30.9	0.0	0.211880	112823	113731	1767-1767	0.333333	51.6
ndhF-rpl32_p5	ndhF-rpl32	5	GGCCGAAAACCACATAGCTA	TCTCAGTCGCGGGGTATAAA	20	20	50.0	50.0	58.0	58.2	0.0	6.1	0.266187	112823	113731	1658-1658	0.333333	51.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCCACCGGACAGTCAAAAAG	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.127156	113906	114546	1090-1099	1.000000	83.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	9.5	0.0	0.107041	113906	114546	1237-1246	1.000000	83.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.162484	113906	114546	739-748	1.000000	83.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.0	58.0	9.5	1.0	0.080892	113906	114546	1357-1366	1.000000	83.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.0	58.0	9.5	1.0	0.080892	113906	114546	1356-1365	1.000000	83.3
ndhE_p1	ndhE	1	AGTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	26	21	26.9	38.1	55.2	54.2	0.0	0.0	13.536278	118367	118687	482-482	0.333333	13.3
ndhE_p2	ndhE	2	GTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	25	21	28.0	38.1	53.9	54.2	0.0	0.0	13.839272	118367	118687	481-481	0.333333	13.3
ndhE_p3	ndhE	3	AGTCTTATGTTTTGATTTCTATGTG	ACTATGGCTCGTCAATAATCT	25	21	28.0	38.1	53.9	54.2	0.0	0.0	13.852333	118367	118687	482-482	0.333333	13.3
ndhE_p4	ndhE	4	AAGTCTTATGTTTTGATTTCTATGTGA	ACTATGGCTCGTCAATAATCT	27	21	25.9	38.1	55.9	54.2	0.0	0.0	13.898888	118367	118687	483-483	0.333333	13.3
ndhE_p5	ndhE	5	AGTCTTATGTTTTGATTTCTATGTGAA	ACTATGGCTCGTCAATAATCT	27	21	25.9	38.1	55.9	54.2	0.0	0.0	13.898888	118367	118687	482-482	0.333333	13.3
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	130050	130616	495-716	1.000000	85.3
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	TTTGAAGGTACTCCCGGGAA	20	20	55.0	50.0	58.0	58.3	0.0	26.7	0.270214	130050	130616	548-769	1.000000	84.6
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CCGTTGTTCCTCAGTAGCTC	TTTTGAAGGTACTCCCGGGA	20	20	55.0	50.0	58.0	58.3	0.0	25.1	0.270214	130050	130616	549-770	1.000000	84.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	15.9	18.9	0.366304	130050	130616	459-680	1.000000	84.3
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	130050	130616	446-667	1.000000	84.3
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	154194	154236	179-179	1.000000	80.5
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154194	154236	178-178	1.000000	80.5
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154194	154236	180-180	1.000000	80.5
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	154194	154236	193-193	1.000000	80.4
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	20	20	55.0	55.0	58.2	58.1	35.0	0.2	0.281592	154194	154236	207-207	1.000000	79.3